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All about western blots
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Proteins: Technique used to detect _____ in a given sample
Electrophoresis: Uses gel ____ to separate, often based on length or size
Antibodies: While on the membrane they are stained with ____ specific to the target
Towbin: This method originated in the lab of Harry ____
Burnette: The name was given to the technique by W. Neal ____
Southern: Western is a play on this other blot technique
Cold: Tissue prep is often done at ___ temperatures to avoid denaturation and degradation
Polyacrylamide: The PA in SDS-PAGE
Wells: Sample is loaded in these
Faster: When separating based on molecular weight the smaller ones move ____ across the gel
Negatively: SDS buffer is ____ charged
Electroblotting: Primary method for transferring product from a gel to a membrane using electric current
Blocking: Step performed to prevent interactions between the membrane and the detection Ab
Probed: During detection the membrane is ____ using Ab’s specific for the target
Unbound: After the detection process membranes are rinsed to remove ____ Ab’s
Secondary: Often ____ Ab’s are used to bind to the primary ones and enhance the signal
Immunoblot: Also known as an ____
Not: Since viruses or environmental sources can provide samples, western blotting is ___ restricted to cellular studies
PonceauS: Sodium salt of a diazo dye that is prepared as a stain for reversible detection of bands
Coomassie: ____ Brilliant Blue dyes are triphenylmethane dyes originally developed for the textile industry but now commonly used to stain for bands
All about western blots
Across:1. | Step performed to prevent interactions between the membrane and the detection Ab | 4. | Primary method for transferring product from a gel to a membrane using electric current | 5. | Uses gel ____ to separate, often based on length or size | 7. | The name was given to the technique by W. Neal ____ | 9. | During detection the membrane is ____ using Ab’s specific for the target |
| 13. | Also known as an ____ | 15. | Technique used to detect _____ in a given sample | 16. | Since viruses or environmental sources can provide samples, western blotting is ___ restricted to cellular studies | 18. | Tissue prep is often done at ___ temperatures to avoid denaturation and degradation | 19. | The PA in SDS-PAGE |
| | Down:2. | SDS buffer is ____ charged | 3. | Western is a play on this other blot technique | 6. | ____ Brilliant Blue dyes are triphenylmethane dyes originally developed for the textile industry but now commonly used to stain for bands | 8. | This method originated in the lab of Harry ____ | 10. | When separating based on molecular weight the smaller ones move ____ across the gel |
| 11. | After the detection process membranes are rinsed to remove ____ Ab’s | 12. | Sample is loaded in these | 14. | While on the membrane they are stained with ____ specific to the target | 15. | Sodium salt of a diazo dye that is prepared as a stain for reversible detection of bands | 17. | Often ____ Ab’s are used to bind to the primary ones and enhance the signal |
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© 2013
PuzzleFast.com, Noncommercial Use Only
All about western blots
Across:1. | Step performed to prevent interactions between the membrane and the detection Ab | 4. | Primary method for transferring product from a gel to a membrane using electric current | 5. | Uses gel ____ to separate, often based on length or size | 7. | The name was given to the technique by W. Neal ____ | 9. | During detection the membrane is ____ using Ab’s specific for the target |
| 13. | Also known as an ____ | 15. | Technique used to detect _____ in a given sample | 16. | Since viruses or environmental sources can provide samples, western blotting is ___ restricted to cellular studies | 18. | Tissue prep is often done at ___ temperatures to avoid denaturation and degradation | 19. | The PA in SDS-PAGE |
| | Down:2. | SDS buffer is ____ charged | 3. | Western is a play on this other blot technique | 6. | ____ Brilliant Blue dyes are triphenylmethane dyes originally developed for the textile industry but now commonly used to stain for bands | 8. | This method originated in the lab of Harry ____ | 10. | When separating based on molecular weight the smaller ones move ____ across the gel |
| 11. | After the detection process membranes are rinsed to remove ____ Ab’s | 12. | Sample is loaded in these | 14. | While on the membrane they are stained with ____ specific to the target | 15. | Sodium salt of a diazo dye that is prepared as a stain for reversible detection of bands | 17. | Often ____ Ab’s are used to bind to the primary ones and enhance the signal |
| |
© 2013
PuzzleFast.com, Noncommercial Use Only