1:00
en
CR
Cell Culture
23
Primary cells: Cells taken directly from a living organism but not immortalized (2 wds)
Serum: Medium supplement that contains a variety of cell growth factors
Aseptic: _________ technique is used to prevent contamination of cell cultures.
Trypsin: A proteolytic enzyme used to dissociate adherent cells
HeLa: Popular cancer cell line isolated from a patient named Henrietta Lacks
Hemacytometer: Microscope slide etched with a grid, often used to count cells
Microscope: Optical instrument used to examine cells in culture
Trypan blue: Dye excluded from viable cells (2 wds)
Biosafety cabinet: Enclosed, ventilated workspace essential to protect personnel from potential pathogens (2 wds)
Medium: Liquid food for cells
EDTA: Divalent cation chelator helpful for dissociating cells
Antibiotics: Media supplements used to prevent the growth of bacteria
Phenol red: pH indicator in media (2 wds)
Nitrogen: Stock cells may be stored in liquid __________.
Ethanol: Alcohol used to decontaminate a work surface before and after cell culture.
Confluent: A cell culture that completely occupies the surface of the flask or dish.
Mycoplasma: Invisible contaminant present in some cell lines
Passaging: Process of transferring cells to fresh growth medium for further propagation
Cryopreservation: Long-term storage of cells in freezing medium
Transfection: Introduction of foreign DNA or RNA into eukaryotic cells by chemical, lipid, or physical means.
Jurkat: Commonly-used T cell line
Endotoxin: QA ensures a low quantity of this in cell-culture grade reagents
Incubator: Enclosed chamber maintained at constant temperature and humidity
Multiwell: Larger scale assays can be performed in _________ plates.
Cell Culture
Across:1. | Divalent cation chelator helpful for dissociating cells | 8. | Enclosed, ventilated workspace essential to protect personnel from potential pathogens (2 wds) | 9. | Commonly-used T cell line | 10. | Larger scale assays can be performed in _________ plates. | 12. | Optical instrument used to examine cells in culture | 15. | Medium supplement that contains a variety of cell growth factors | 16. | A proteolytic enzyme used to dissociate adherent cells | 18. | Alcohol used to decontaminate a work surface before and after cell culture. | 19. | Enclosed chamber maintained at constant temperature and humidity | 21. | Stock cells may be stored in liquid __________. | 22. | pH indicator in media (2 wds) | 23. | Media supplements used to prevent the growth of bacteria |
| | Down:2. | Dye excluded from viable cells (2 wds) | 3. | Popular cancer cell line isolated from a patient named Henrietta Lacks | 4. | Invisible contaminant present in some cell lines | 5. | Cells taken directly from a living organism but not immortalized (2 wds) | 6. | _________ technique is used to prevent contamination of cell cultures. | 7. | Microscope slide etched with a grid, often used to count cells | 11. | A cell culture that completely occupies the surface of the flask or dish. | 13. | QA ensures a low quantity of this in cell-culture grade reagents | 14. | Process of transferring cells to fresh growth medium for further propagation | 17. | Introduction of foreign DNA or RNA into eukaryotic cells by chemical, lipid, or physical means. | 20. | Liquid food for cells |
| |
© 2013
PuzzleFast.com, Noncommercial Use Only
Cell Culture
Across:1. | Divalent cation chelator helpful for dissociating cells | 8. | Enclosed, ventilated workspace essential to protect personnel from potential pathogens (2 wds) | 9. | Commonly-used T cell line | 10. | Larger scale assays can be performed in _________ plates. | 12. | Optical instrument used to examine cells in culture | 15. | Medium supplement that contains a variety of cell growth factors | 16. | A proteolytic enzyme used to dissociate adherent cells | 18. | Alcohol used to decontaminate a work surface before and after cell culture. | 19. | Enclosed chamber maintained at constant temperature and humidity | 21. | Stock cells may be stored in liquid __________. | 22. | pH indicator in media (2 wds) | 23. | Media supplements used to prevent the growth of bacteria |
| | Down:2. | Dye excluded from viable cells (2 wds) | 3. | Popular cancer cell line isolated from a patient named Henrietta Lacks | 4. | Invisible contaminant present in some cell lines | 5. | Cells taken directly from a living organism but not immortalized (2 wds) | 6. | _________ technique is used to prevent contamination of cell cultures. | 7. | Microscope slide etched with a grid, often used to count cells | 11. | A cell culture that completely occupies the surface of the flask or dish. | 13. | QA ensures a low quantity of this in cell-culture grade reagents | 14. | Process of transferring cells to fresh growth medium for further propagation | 17. | Introduction of foreign DNA or RNA into eukaryotic cells by chemical, lipid, or physical means. | 20. | Liquid food for cells |
| |
© 2013
PuzzleFast.com, Noncommercial Use Only