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All About PCR
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Polymerase: Enzyme that synthesizes a DNA strand from a template
Thermocycler: Equipment that goes through repeated cycles of temperature changes
Primers: Short oligonucleotides used as the starting point for DNA synthesis
Template: Target DNA to be amplified
Taq: Polymerase isolated from the bacterium Thermus aquaticus
dNTPs: building blocks for DNA synthesis
Denaturation: Temperature-mediated separation of the DNA double helix
Annealing: Stage in which the primers bind to the DNA target
Reverse Transcriptase: Enzyme responsible for transcribing RNA into cDNA (2 wds)
RTPCR: Amplification of DNA from RNA
Quantitative: q, in qPCR
Mullis: PCR inventor
Nested: A variation of the PCR technique in which two sets of primers are used successively
Exonuclease: High-fidelity polymerases possess 3’ to 5’ __________ activity.
Tm: Primer GC content often increases this
Hairpins: Dimers and these can result in reduced primer efficiency
Magnesium: Cofactor ions used in PCR reactions
Hot start: PCR variation that involves heating the reaction before adding the polymerase (2 wds)
Reaction: Polymerase chain _____________
Pfu: Proof-reading polymerase isolated from Pyrococcus furiosus
Amplicon: DNA product generated by PCR
Agarose gel: Following PCR, the quality of the product is often assessed by this (2 wds)
Fluorescent: ________ dyes, such as SybrGreen, are often used to determine the amount of product in real time.
All About PCR
Across:1. | q, in qPCR | 3. | Stage in which the primers bind to the DNA target | 7. | PCR inventor | 8. | Dimers and these can result in reduced primer efficiency | 10. | PCR variation that involves heating the reaction before adding the polymerase (2 wds) | 11. | Short oligonucleotides used as the starting point for DNA synthesis | 14. | Target DNA to be amplified | 15. | Enzyme that synthesizes a DNA strand from a template | 16. | Proof-reading polymerase isolated from Pyrococcus furiosus | 17. | Primer GC content often increases this | 19. | Polymerase chain _____________ | 20. | A variation of the PCR technique in which two sets of primers are used successively | 22. | High-fidelity polymerases possess 3’ to 5’ __________ activity. |
| | Down:2. | Following PCR, the quality of the product is often assessed by this (2 wds) | 4. | Equipment that goes through repeated cycles of temperature changes | 5. | ________ dyes, such as SybrGreen, are often used to determine the amount of product in real time. | 6. | building blocks for DNA synthesis | 9. | DNA product generated by PCR | 12. | Amplification of DNA from RNA | 13. | Temperature-mediated separation of the DNA double helix | 18. | Cofactor ions used in PCR reactions | 21. | Polymerase isolated from the bacterium Thermus aquaticus |
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© 2013
PuzzleFast.com, Noncommercial Use Only
All About PCR
Across:1. | q, in qPCR | 3. | Stage in which the primers bind to the DNA target | 7. | PCR inventor | 8. | Dimers and these can result in reduced primer efficiency | 10. | PCR variation that involves heating the reaction before adding the polymerase (2 wds) | 11. | Short oligonucleotides used as the starting point for DNA synthesis | 14. | Target DNA to be amplified | 15. | Enzyme that synthesizes a DNA strand from a template | 16. | Proof-reading polymerase isolated from Pyrococcus furiosus | 17. | Primer GC content often increases this | 19. | Polymerase chain _____________ | 20. | A variation of the PCR technique in which two sets of primers are used successively | 22. | High-fidelity polymerases possess 3’ to 5’ __________ activity. |
| | Down:2. | Following PCR, the quality of the product is often assessed by this (2 wds) | 4. | Equipment that goes through repeated cycles of temperature changes | 5. | ________ dyes, such as SybrGreen, are often used to determine the amount of product in real time. | 6. | building blocks for DNA synthesis | 9. | DNA product generated by PCR | 12. | Amplification of DNA from RNA | 13. | Temperature-mediated separation of the DNA double helix | 18. | Cofactor ions used in PCR reactions | 21. | Polymerase isolated from the bacterium Thermus aquaticus |
| |
© 2013
PuzzleFast.com, Noncommercial Use Only