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All About PCR

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Across:

1.	q, in qPCR
3.	Stage in which the primers bind to the DNA target
7.	PCR inventor
8.	Dimers and these can result in reduced primer efficiency
10.	PCR variation that involves heating the reaction before adding the polymerase (2 wds)
11.	Short oligonucleotides used as the starting point for DNA synthesis
14.	Target DNA to be amplified
15.	Enzyme that synthesizes a DNA strand from a template
16.	Proof-reading polymerase isolated from Pyrococcus furiosus
17.	Primer GC content often increases this
19.	Polymerase chain _____________
20.	A variation of the PCR technique in which two sets of primers are used successively
22.	High-fidelity polymerases possess 3’ to 5’ __________ activity.

Down:

2.	Following PCR, the quality of the product is often assessed by this (2 wds)
4.	Equipment that goes through repeated cycles of temperature changes
5.	________ dyes, such as SybrGreen, are often used to determine the amount of product in real time.
6.	building blocks for DNA synthesis
9.	DNA product generated by PCR
12.	Amplification of DNA from RNA
13.	Temperature-mediated separation of the DNA double helix
18.	Cofactor ions used in PCR reactions
21.	Polymerase isolated from the bacterium Thermus aquaticus

© 2013 PuzzleFast.com, Noncommercial Use Only

All About PCR

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Across:

1.	q, in qPCR
3.	Stage in which the primers bind to the DNA target
7.	PCR inventor
8.	Dimers and these can result in reduced primer efficiency
10.	PCR variation that involves heating the reaction before adding the polymerase (2 wds)
11.	Short oligonucleotides used as the starting point for DNA synthesis
14.	Target DNA to be amplified
15.	Enzyme that synthesizes a DNA strand from a template
16.	Proof-reading polymerase isolated from Pyrococcus furiosus
17.	Primer GC content often increases this
19.	Polymerase chain _____________
20.	A variation of the PCR technique in which two sets of primers are used successively
22.	High-fidelity polymerases possess 3’ to 5’ __________ activity.

Down:

2.	Following PCR, the quality of the product is often assessed by this (2 wds)
4.	Equipment that goes through repeated cycles of temperature changes
5.	________ dyes, such as SybrGreen, are often used to determine the amount of product in real time.
6.	building blocks for DNA synthesis
9.	DNA product generated by PCR
12.	Amplification of DNA from RNA
13.	Temperature-mediated separation of the DNA double helix
18.	Cofactor ions used in PCR reactions
21.	Polymerase isolated from the bacterium Thermus aquaticus

© 2013 PuzzleFast.com, Noncommercial Use Only