Make everything smaller

Make everything bigger

All about western blots

										1						2				3

		4

												5

														6
7						8						9

											10
								11									12

		13

		14								15
		16

						17				18

	19

Across:

1.	Step performed to prevent interactions between the membrane and the detection Ab
4.	Primary method for transferring product from a gel to a membrane using electric current
5.	Uses gel ____ to separate, often based on length or size
7.	The name was given to the technique by W. Neal ____
9.	During detection the membrane is ____ using Ab’s specific for the target

13.	Also known as an ____
15.	Technique used to detect _____ in a given sample
16.	Since viruses or environmental sources can provide samples, western blotting is ___ restricted to cellular studies
18.	Tissue prep is often done at ___ temperatures to avoid denaturation and degradation
19.	The PA in SDS-PAGE

Down:

2.	SDS buffer is ____ charged
3.	Western is a play on this other blot technique
6.	____ Brilliant Blue dyes are triphenylmethane dyes originally developed for the textile industry but now commonly used to stain for bands
8.	This method originated in the lab of Harry ____
10.	When separating based on molecular weight the smaller ones move ____ across the gel

11.	After the detection process membranes are rinsed to remove ____ Ab’s
12.	Sample is loaded in these
14.	While on the membrane they are stained with ____ specific to the target
15.	Sodium salt of a diazo dye that is prepared as a stain for reversible detection of bands
17.	Often ____ Ab’s are used to bind to the primary ones and enhance the signal

© 2013 PuzzleFast.com, Noncommercial Use Only

All about western blots

1

B

L

O

C

K

I

2

N

G

3

S

E

O

4

E

L

E

C

T

R

O

B

L

O

T

T

I

N

G

U

A

T

5

E

L

E

C

T

R

O

P

H

O

R

E

S

I

S

I

E

6

C

V

R

7

B

U

R

N

E

T

8

T

E

9

P

R

O

B

E

D

N

O

O

L

W

10

F

M

Y

B

11

U

A

A

12

W

I

N

S

S

E

13

I

M

M

U

N

O

B

L

O

T

S

L

O

E

I

L

14

A

U

15

P

R

O

T

E

I

N

S

16

N

O

T

N

O

T

D

N

I

17

S

18

C

O

L

D

B

E

E

19

P

O

L

Y

A

C

R

Y

L

A

M

I

D

E

D

O

U

I

N

S

E

D

S

A

R

Y

Across:

1.	Step performed to prevent interactions between the membrane and the detection Ab
4.	Primary method for transferring product from a gel to a membrane using electric current
5.	Uses gel ____ to separate, often based on length or size
7.	The name was given to the technique by W. Neal ____
9.	During detection the membrane is ____ using Ab’s specific for the target

13.	Also known as an ____
15.	Technique used to detect _____ in a given sample
16.	Since viruses or environmental sources can provide samples, western blotting is ___ restricted to cellular studies
18.	Tissue prep is often done at ___ temperatures to avoid denaturation and degradation
19.	The PA in SDS-PAGE

Down:

2.	SDS buffer is ____ charged
3.	Western is a play on this other blot technique
6.	____ Brilliant Blue dyes are triphenylmethane dyes originally developed for the textile industry but now commonly used to stain for bands
8.	This method originated in the lab of Harry ____
10.	When separating based on molecular weight the smaller ones move ____ across the gel

11.	After the detection process membranes are rinsed to remove ____ Ab’s
12.	Sample is loaded in these
14.	While on the membrane they are stained with ____ specific to the target
15.	Sodium salt of a diazo dye that is prepared as a stain for reversible detection of bands
17.	Often ____ Ab’s are used to bind to the primary ones and enhance the signal

© 2013 PuzzleFast.com, Noncommercial Use Only