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RT-PCR
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Reverse : _______ Transcription: Instead of making new DNA from a template of DNA, make DNA from RNA. (In other words, convert mRNA into cDNA). (7)
Polymerase : P in RT-PCR stands for _______. (10)
Template : cDNA product as a _______ for the PCR reaction. (8)
Amplicon : The portion of the gene that is amplified is called the ____________. (8)
Enzyme : Each reaction of a master mixes contain a buffer and an _______. (6)
Transcriptase : Enzyme reverse _______ will be used to synthesize cDNA from the mRNA isolated in previous lab session. (13)
GFP : PCR amplify ____ gene. (3)
Inactivate : Incubation at 70°C for 15 minutes ________ the reaction. (10)
SuperScript : The ________III RT needs to be added to the cDNA Synthesis Mix before you use the Mix. (11)
Anneal : PCR involves repeated cycles of 3 step process: denature (heat), anneal primers (cool), ______ strand (Warm). (8)
Primers : _______ provide starting point for DNA polymerase. (7)
cDNA : Before we can get to PCR, what do you have to do? Make _____from RNA. (4)
Fluorescence : The thermocycler measures the amount of _________at each step. (12)
Six : Each group will have ____ (a number) RT reactions. (3)
Eight : Each group will have _____ (a number) PCR reactions. (5)
RT-PCR
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Z | B | A | E | B | K | X | H | B | E | T | K | Q | A | K | F | E | G | N | D |
P | L | K | J | K | S | R | D | S | G | H | U | N | A | T | M | T | T | N | K |
J | T | T | O | X | X | C | A | N | C | C | D | N | S | Y | Z | E | B | O | B |
A | N | N | E | A | L | R | J | C | H | C | D | R | V | C | S | N | E | C | G |
D | D | F | S | F | E | R | F | Z | R | H | E | W | G | I | E | T | S | I | Q |
N | P | Q | C | M | Z | M | F | H | A | M | P | V | X | H | S | S | A | L | G |
R | M | V | Y | I | J | A | J | W | I | K | E | M | Y | Z | N | E | T | P | Y |
C | Z | L | G | P | T | V | L | R | K | P | R | L | E | T | J | A | P | M | L |
R | O | F | U | E | U | V | P | S | A | E | I | P | R | P | P | V | I | A | R |
P | D | S | W | W | T | U | B | L | D | N | P | O | G | I | U | C | R | T | T |
L | X | O | A | B | A | A | E | V | A | X | X | F | M | R | D | D | C | V | Y |
P | B | E | V | P | Q | J | L | C | Z | T | P | P | N | C | H | P | S | X | P |
G | F | K | I | I | F | J | T | P | E | I | G | H | T | S | W | B | N | M | B |
W | X | J | H | B | B | I | X | L | M | Y | Y | N | C | R | R | A | A | W | U |
W | U | F | B | S | V | U | U | K | E | E | Z | C | X | E | J | R | R | K | X |
D | O | S | K | A | U | D | O | N | K | C | T | G | M | P | H | Y | T | F | Q |
Z | D | L | T | H | N | L | V | T | C | F | K | Q | J | U | H | B | J | O | U |
D | B | E | T | J | Z | Q | E | S | R | E | V | E | R | S | Q | N | I | A | A |
V | S | B | I | J | W | R | P | A | J | O | S | S | R | H | G | U | R | R | H |
- PCR amplify ____ gene. (3)
- Each group will have ____ (a number) RT reactions. (3)
- Before we can get to PCR, what do you have to do? Make _____from RNA. (4)
- Each group will have _____ (a number) PCR reactions. (5)
- PCR involves repeated cycles of 3 step process: denature (heat), anneal primers (cool), ______ strand (Warm). (8)
- Each reaction of a master mixes contain a buffer and an _______. (6)
- _______ provide starting point for DNA polymerase. (7)
- _______ Transcription: Instead of making new DNA from a template of DNA, make DNA from RNA. (In other words, convert mRNA into cDNA). (7)
- The portion of the gene that is amplified is called the ____________. (8)
- cDNA product as a _______ for the PCR reaction. (8)
- Incubation at 70°C for 15 minutes ________ the reaction. (10)
- P in RT-PCR stands for _______. (10)
- The ________III RT needs to be added to the cDNA Synthesis Mix before you use the Mix. (11)
- The thermocycler measures the amount of _________at each step. (12)
- Enzyme reverse _______ will be used to synthesize cDNA from the mRNA isolated in previous lab session. (13)
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PuzzleFast.com, Noncommercial Use Only
RT-PCR
W | J | J | A | H | E | F | L | U | O | R | E | S | C | E | N | C | E | F | W |
Z | B | A | E | B | K | X | H | B | E | T | K | Q | A | K | F | E | G | N | D |
P | L | K | J | K | S | R | D | S | G | H | U | N | A | T | M | T | T | N | K |
J | T | T | O | X | X | C | A | N | C | C | D | N | S | Y | Z | E | B | O | B |
A | N | N | E | A | L | R | J | C | H | C | D | R | V | C | S | N | E | C | G |
D | D | F | S | F | E | R | F | Z | R | H | E | W | G | I | E | T | S | I | Q |
N | P | Q | C | M | Z | M | F | H | A | M | P | V | X | H | S | S | A | L | G |
R | M | V | Y | I | J | A | J | W | I | K | E | M | Y | Z | N | E | T | P | Y |
C | Z | L | G | P | T | V | L | R | K | P | R | L | E | T | J | A | P | M | L |
R | O | F | U | E | U | V | P | S | A | E | I | P | R | P | P | V | I | A | R |
P | D | S | W | W | T | U | B | L | D | N | P | O | G | I | U | C | R | T | T |
L | X | O | A | B | A | A | E | V | A | X | X | F | M | R | D | D | C | V | Y |
P | B | E | V | P | Q | J | L | C | Z | T | P | P | N | C | H | P | S | X | P |
G | F | K | I | I | F | J | T | P | E | I | G | H | T | S | W | B | N | M | B |
W | X | J | H | B | B | I | X | L | M | Y | Y | N | C | R | R | A | A | W | U |
W | U | F | B | S | V | U | U | K | E | E | Z | C | X | E | J | R | R | K | X |
D | O | S | K | A | U | D | O | N | K | C | T | G | M | P | H | Y | T | F | Q |
Z | D | L | T | H | N | L | V | T | C | F | K | Q | J | U | H | B | J | O | U |
D | B | E | T | J | Z | Q | E | S | R | E | V | E | R | S | Q | N | I | A | A |
V | S | B | I | J | W | R | P | A | J | O | S | S | R | H | G | U | R | R | H |
- GFP
- SIX
- CDNA
- EIGHT
- ANNEAL
- ENZYME
- PRIMERS
- REVERSE
- AMPLICON
- TEMPLATE
- INACTIVATE
- POLYMERASE
- SUPERSCRIPT
- FLUORESCENCE
- TRANSCRIPTASE
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© 2015
PuzzleFast.com, Noncommercial Use Only